Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Instrukcja Użytkownika Pobierz pdf (Strona 10)

yeast for processing. To calculate the volume of culture required, use the following

equation:

(OD

600

of undiluted culture) x (culture volume in ml) = # OD•ml

For example, 3 OD•ml of yeast would require 500 µl of an undiluted culture with an

600

= 6.

Note: 1 OD

600

is equivalent to approximately 8 x 10

bacterial cells/ml, or

1 x 10

yeast cells/ml.

Table 2. Yield (per column) of total RNA from various samples using

the Aurum total RNA fatty and fibrous tissue kit.

Average Yield

Starting Material (Amount Used)* (µg)**

Cultured cells (1 x 10

)

293H 145

Bacteria (2.4 x 10

or 3 OD•ml)

E. coli 30

Yeast (3 x 10

or 3 OD•ml)

S. cerevisiae 64

Fatty animal tissue (100 mg)

Brain 96

Breast 58

Adipose 14

Fibrous animal tissue (100 mg)

Heart 85

Cartilage 54

Skin 63

Plant tissue (100 mg)

Potato 91

Arabidopsis 5

Filamentous fungi (50 mg)

Aspergillus niger 12

* Starting material amounts in parentheses are the maximum amounts recommended for this kit. Note: The

elution volume should be decreased to 30 µl if only a small amount of starting material (<500,000 cells or

<10 mg of animal or plant tissue) is used.

** Yield figures are representative of a minimum of 20 mini column preps performed in both vacuum and spin

formats.

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