Bio-rad Biofuel Enzyme Kit Instrukcja Użytkownika Pobierz pdf (Strona 10)

Quick Guide

Activity 6: Test the Ability of

Mushroom Extracts to Increase

the Reaction Rate

1. Write down the name of your mushroom

_______________________

2. Weigh out approximately 1 gram of

mushroom and put it into a mortar.

3. Add 2 ml of extraction buffer for every

gram of mushroom into the mortar. To

calculate the amount of extraction buffer

you need, multiply the weight (in grams)

of the mushroom by 2 and add that many

milliliters.

Weight of mushroom ____ g x 2 = _____ ml

4. Using a pestle, grind your mushroom to

produce a slurry.

5. Strain the solid particles out of your slurry

using a piece of filter paper or cheese

cloth into a 1.5 ml microcentrifuge tube.

Alternatively, if you have a centrifuge,

scoop the slurry into a 1.5 ml

microcentrifuge tube and then pellet the

solid particles by spinning at top speed

for 2 minutes. Note: You will need at

least 250 µl of extract to perform the

enzymatic reaction.

6. Label your cuvettes “1–6”. Only label on

the upper part of the cuvette face.

35

QUICK GUIDE

Quick Guide

Extraction buffer

Label up here

1 2 ... 5 6 7 8 9 10 11 12

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