Bio-rad Comparative Proteomics Kit I: Protein Profiler Mod Instrukcja Użytkownika Pobierz pdf (Strona 3)

11. Heat fish samples and actin and myosin

standard to 95°C for 2–5 min.

12. Load your gel:

Lane Volume Sample

1 & 2 empty empty

3 5 µl Precision Plus Protein Kaleidoscope

prestained standards (Stds)

4 10 µl fish sample 1

5 10 µl fish sample 2

6 10 µl fish sample 3

7 10 µl fish sample 4

8 10 µl fish sample 5

9 10 µl actin and myosin standard

(AM)

10 empty empty

13. Electrophorese for 30 minutes at 200 V in 1x

TGS electrophoresis buffer.

14. After electrophoresis, remove gel from cassette

and transfer gel to a container with 25 ml Bio-Safe

Coomassie blue stain per gel and stain gel for

1 hour, with gentle shaking for best results.

Lesson 3 Quick Guide

1. Discard stain and destain gels in a large

volume of water for at least 30 minutes to overnight,

changing the water at least once. Blue-stained

bands will be visible on a clear gel after destaining.

2. Dry gels using GelAir cellophane.

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Bio-rad Comparative Proteomics Kit I: Protein Profiler Mod Instrukcja Użytkownika Strona 3