Bio-rad ENrich™ High-Resolution Ion Exchange Columns Instrukcja Użytkownika Pobierz pdf (Strona 8)

4 ENrich Instruction Manual

Table 2. Buffers for ion exchange chromatography.

Anion Exchange — ENrich Q

Range

Buffer MW pKa @

25°C

Counter-Ion pKa/°C

5.0–6.0 Piperazine 86.1 5.7 Cl-/HCOO

–

–0.015

5.0–6.0 L-histidine 155.2 6.15 CI

–

5.8–7.2 Bis-Tris 209.2 6.5 CI

–

–0.017

6.4–7.3 Bis-Tris propane 282.3 6.8,9 CI

–

7.3–8.3 Triethanolamine 149.2 7.8 CI-/CH3COO

–

–0.02

7.6–8.6 Tris 121.1 8.1 CI

–

–0.031

8.4–8.8 Diethanolamine 105.1 8.9 CI

–

–0.025

9.0–9.9 Ethanolamine 61.1 9.5 CI

–

–0.029

9.8–10.3 1,3-diamino-

propane

74.1 10.5 CI

–

–0.023

Cation Exchange — ENrich S

Range

Buffer MW pKa @

25°C

Counter-Ion pKa/°C

3.6–4.3 Lactic acid 90.1 3.8 Na

–

4.2–5.2 Citric acid 192.1 3.1 Na

–

5.5–6.7 MES 195.2 6.1 Na

–0.011

6.1–7.5 PIPES 302.4 6.8 Na

–0.009

6.5–7.9 MOPS 209.3 7.2 Na

–0.006

6.7–7.6 Phosphate 120/142 7.2 Na

–0.003

6.8–8.2 TES 229.2 7.4 Na

–0.002

6.8–8.2 HEPS 238.3 7.5 Na

–0.014

7.4–8.8 Tricine 179.2 8.1 Na

–0.021

Always use buffer components of the highest purity available,

as UV-absorbing impurities may cause baseline disturbances

and interfere with the detection of protein peaks. Always ﬁlter

and degas buffers.

1 2 3 4 5 6 7 8 9 10 11 12 13 ... 19 20

Komentarze do niniejszej Instrukcji

Brak uwag

Bio-rad ENrich™ High-Resolution Ion Exchange Columns Instrukcja Użytkownika Strona 8

Komentarze do niniejszej Instrukcji

Powiązane produkty i podręczniki dla Urządzenia pomiarowe Bio-rad ENrich™ High-Resolution Ion Exchange Columns