Bio-rad Microplate Manager Software Instrukcja Użytkownika Pobierz pdf (Strona 44)

Analyzing Data Using a Standard Curve

Log/log Fit

The Log/Log curve fit option fits the best straight line to the data consisting of the

logarithm of the OD (response) vs. the logarithm of the concentration.

For the Log/log method, the equation for the standard curve is:

Log

(y) =Intercept + Slope*Log

(conc)

In the plot, the concentration and the OD axes are displayed on the log scale. The

calculated intercept, slope, the correlation coefficient (r) and the chi

values of the

standard curve are shown below the plot.

The log-log option should be used when a linear plot of the data exhibits a strong

curvature up or down.

Quadratic

The quadratic function fits the best parabolic curve according to the equation

Y = A + Bx + Cx

where A is the intercept, B is the slope of the curve at the intercept, and C is the

measure of curvature of the parabola.

The quadratic fit is most appropriate when the standard curve curves up or down.

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Bio-rad Microplate Manager Software Instrukcja Użytkownika Strona 44