Bio-rad PV92 PCR Informatics Kit Instrukcja Użytkownika Pobierz pdf (Strona 2)

9. When all members of your team have

collected their samples, place the tubes in the

foam micro test-tube holder, and incubate at

56°C for 10 minutes in a water bath. At the

halfway point (5 minutes), shake or vortex the

tubes gently, then place back in the 56°C water

bath for the remaining 5 minutes.

10. Remove the tubes, shake or vortex, and place

the tubes in a boiling water bath (100°C).

Incubate at 100°C for 5 minutes.

11. Remove the tubes from the boiling water bath

and shake or vortex the contents to resuspend.

Pellet the matrix by spinning at 6,000 x g for

5 minutes (or 2,000 x g for 10 minutes) in a

centrifuge.

12. Store your screwcap tube in the refrigerator until

the next laboratory period (or proceed to step 2

of Lesson 2).

Centrifuge

56°C, 10 min

100°C, 5 min

Water bath

1 2 3 4 5 6 7

Brak uwag

Bio-rad PV92 PCR Informatics Kit Instrukcja Użytkownika Strona 2