Bio-rad RC DC™ Protein Assay Instrukcja Użytkownika Pobierz pdf (Strona 5)

Note: Also see DCProtein Assay Instruction Manual for additional

troubleshooting recommendations.

Questions

1 May I use a wavelength other than

750 nm?

2 What should I do if the protein

pellet is still soft after centrifugation

for 3 to 5 minutes?

3 What can I do to minimize

interference from supernatant

carry-over?

Recommendations

Yes, absorbance can be measured at

650-750 nm.

Increase the centrifugation duration to

6-10 minutes. Protein pellet may take

longer to dissolve after the addition of

Reagent A´.

Option #1:

A second wash can be performed as

follows:

Standard Assay

After step #6, repeat step #4 with

500 µl of Reagent I, repeat step #5

with 160 µl of Reagent II, repeat

step #6 before going on to step #7.

Microfuge Tube Assay

After step #6, repeat step #4 with

125 µl of Reagent I, repeat step #5

with 40 µl of Reagent II, repeat step

#6 before going on to step #7.

Option #2:

At step #6: to maximize supernatant

removal, discard supernatant by

aspiration before going on to step #7.

Option #3:

After step #6, dry tubes under

vacuum to reduce residual

supernatant before going on to

step #7.

Section 6 Troubleshooting Guide

4110107A.qxd 08/29/2000 11:38 AM Page 4

1 2 3 4 5 6 7 8 9 10

Brak uwag

Bio-rad RC DC™ Protein Assay Instrukcja Użytkownika Strona 5