
Table of Contents
Section 1 Introduction.............................1
Section 2 Kit Components .........................1
Section 3 Storage Conditions .......................2
Section 4 Necessary Supplies.......................2
Section 5 Before Using the
Aurum™ Total RNA 96 Kit...................3
Starting Material Amounts .......................3
Reagents Used With the Aurum™ Total RNA 96 Kit ...4
Elution Guidelines ..............................5
Ribonucleases ................................5
Disruption and Homogenization ...................6
Section 6 Vacuum Manifold Setup and Use With
96-Well Plates...........................6
Guidelines for Vacuum Format ....................6
Preparing the Aurum™ Vacuum Manifold............7
Vacuum Setup ................................7
Manifold Wash Setup ...........................7
Manifold Elution Setup ..........................8
Section 7 Vacuum Protocol.........................9
Section 8 Spin Protocol ..........................12
Section 9 Troubleshooting Guide ...................15
Section 10 Ordering Information.....................19
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