Bio-rad Bio-Scale™ MT High-Resolution Empty Columns Instrukcja Użytkownika Pobierz pdf (Strona 11)

Bio-Scale MT Column Instruction Manual 7

8. Pass 5 bed volumes of buffer through the column at a ﬂow

rate 20% higher than the operating ﬂow rate.

9. If necessary, stop the ﬂow and lower the upper bed support

via the lock nut to remove any head space that might have

formed between the frit and the top of the bed.

10. Equilibrate the column at the desired ﬂow rate.

2.4 Connections to Chromatography Systems

Bio-Scale MT columns can be used with any chromatography

system that can provide the appropriate ﬂow rate and pressure.

Each column is supplied with 1/4-28 ﬁttings for connection to

Bio-Rad’s BioLogic System, M6 ﬁttings for connection to FPLC®

systems, and 10-32 ﬁttings for connection to HPLC systems. Inlet

and outlet tubing must be 1/16” OD. Inner diameters of 0.020” or

0.030” in Tefzel®, PTFE or PEEK material are recommended.

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Bio-rad Bio-Scale™ MT High-Resolution Empty Columns Instrukcja Użytkownika Strona 11