Bio-rad Quantum Prep Plasmid Kits Instrukcja Użytkownika Strona 1

Quantum Prep® Plasmid Miniprep KitInstruction ManualCatalog #732-6100For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-424

The final formulation of the wash buffer contains 50% ethanol. To achieve this, add 63 ml of ethanol to the wash buffer before first use of the Quantu

2.3 Helpful Hints1. It is recommended that the cells do not sit longer than five minutes at step 3 (lysis) before proceeding to step 4. Additionally,

830252015105045040035030025020015010050Elution volume, µlDNA yieldDNA concentration025DNA concentration, ng/µlDNA yield, µg5075100125150175200Fig. 1.

Section 3 References1. U.S. Patent 5,075,430 issued to Bio-Rad Laboratories.2. Ausubel et al, Current Protocols in Molecular Biology, Wiley-Intersci

Life ScienceGroup11-1090 0711 Sig 02114100066 Rev G US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61

Table of Contents Section 1 Introduction ... 1 1.1 Overview ...

Section 1 Introduction1.1 OverviewThe original alkaline lysis method for purifying plasmid DNA from bacterial cultures requires organic reagents and t

Plasmid yield depends on a number of factors such as the vector copy number, culture volume and aeration, bacterial strain, and growth media. The Quan

1.3 Storage and StabilityAll components are stable for 12 months from the date of purchase when stored at room temperature and used as described in th

Tris-HCl, 0.1 mM EDTA, pH 8.0). Eluting with H2O or TE preheated to 70°C can also improve the yield. However, these high temperatures may partially de

should become viscous and slightly clear if cell lysis has occurred.4. Add 250 µl of the neutralization solution and mix by gently inverting the capp