Bio-rad Quantum Prep Plasmid Kits Instrukcja Użytkownika Pobierz pdf (Strona 6)

Plasmid yield depends on a number of factors such as the

vector copy number, culture volume and aeration, bacterial strain,

and growth media. The Quantum Prep kit was optimized using

high copy number plasmids grown in rich media such as Terrific

Broth.

The Quantum Prep kit permits yields as high as 25 µg from

a 1.5 ml culture of DH5αF'[pTZ18U] grown in Terrific Broth. Yields

as high as 40 µg have been obtained with other plasmids using

the Quantum Prep kit.

1.2 Kit Contents

The Quantum Prep Miniprep kit contains reagents sufficient for

100 plasmid minipreps.

25 ml Cell Resuspension Solution

25 ml Cell Lysis Solution

25 ml Neutralization Solution

20 ml Quantum Prep Matrix

63 ml Wash Buffer

100 Spin Filters

100 Wash Tubes

100 Collection Tubes

1 2 3 4 5 6 7 8 9 10 11 ... 15 16

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Bio-rad Quantum Prep Plasmid Kits Instrukcja Użytkownika Strona 6