Bio-rad Aurum™ Total RNA Mini Kit Instrukcja Użytkownika Pobierz pdf (Strona 24)

Problem Possible Cause Recommended Solution

Low eluate volume Insufficient centrifugation Add 1–3 min to the

(<60 µl) time during elution centrifugation time during

elution

Column clogging See problem “Clogging

of RNA binding column”

High eluate volume Low stringency wash Add 1–3 min to the

(>80 µl) carryover in eluate centrifugation time

after the final wash step

Low RNA yield Low amount of Increase starting

starting material material amount up to

the maximum indicated

for the specific starting

material type

Excessive amount of Reduce amount of

starting material starting material used

Poor disruption and/or Increase intensity/

homogenization duration of disruption

and/or homogenization

Switch to more intense

disruption and/or

homogenization method

Incorrect use of wash Add the appropriate

solutions volume of 95–100%

ethanol to the wash

solutions before initial

use

Incorrect preparation of Use only the DNase

DNase dilution dilution solution

provided in the kit

to dilute the DNase

Low sample eluate See problem “Low

volume eluate volumes (<60 µl)”

Inefficient elution Preheat the elution

solution to 70°C in

water bath prior to

the elution step

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Bio-rad Aurum™ Total RNA Mini Kit Instrukcja Użytkownika Strona 24