Bio-rad Aurum™ Total RNA Mini Kit Instrukcja Użytkownika Pobierz pdf (Strona 9)

Reagents Used With the Aurum Total RNA Mini Kit

• The low stringency wash solution is provided as a 5x concentrate. Add

4 volumes (80 ml) of 95–100% ethanol to the low stringency

wash solution concentrate before initial use

• Before using the lysis solution, add 500 µl of b-mercaptoethanol

(catalog # 161-0710) to the solution for a final concentration of 1%

• The RNase-free DNase I is provided as a lyophilized powder. Reconstitute

the DNase I by adding 250 µl 10 mM Tris, pH 7.5 (not supplied) to the vial

and pipetting up and down briefly to mix. Do not vortex. Store the

reconstituted DNase I at –20°C in a nonfrost-free freezer

• Bacterial total RNA isolations require the use of TE (10 mM Tris,

1 mM EDTA, pH 7.5), which is not supplied with the kit

• Yeast total RNA isolations require the use of lyticase dilution buffer

(1 M sorbitol, 0.1 M EDTA, pH 7.4, 0.1% b-mercaptoethanol), which is

not supplied with the kit

• Vendors of lyticase, which is used to partially degrade the cell walls of yeast

cells, may have different definitions of the enzyme’s activity. As used in this

instruction manual, 1 unit of lyticase produces a DA

800

of 0.001/min at

pH 7.5 at 25°C, using 3 ml of yeast suspension as a substrate in a 3 ml

reaction volume

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Bio-rad Aurum™ Total RNA Mini Kit Instrukcja Użytkownika Strona 9