Bio-rad Profinity eXact™ Purification and Tag Cleavage Con Instrukcja Użytkownika Strona 3

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Section 1
Introduction
Bio-Scale Mini cartridges are convenient, disposable,
prepacked chromatography cartridges. Patented*
column design assures leak-free operation with any
low pressure chromatography system. Bio-Scale
Mini cartridges are available for a variety of
chromatographic techniques such as desalting, ion
exchange, hydrophobic interaction, and affinity
chromatography.
Bio-Scale Mini Profinity eXact cartridges are packed
with Profinity eXact (EX
act Affinity Cleavage T
echnology)
purification resin. This agarose-based affinity
chromatography resin utilizes an immobilized, modified
protease that selectively binds the fusion protein
and cleaves the affinity tag on-column under
controlled conditions, releasing the purified target
protein which contains only its native amino acid
sequence. This innovative resin technology improves
the efficiency of recombinant protein purification, and
is the only affinity chromatography platform that
completes the purification and tag removal process in
a single step
.
*U.S. patent 7,208,087
1
Section 9 Scaling Up........................................23
Section 10 Regenerating, Cleaning,
and Storage .....................................23
Section 11 Troubleshooting Guide......................26
Section 12 Ordering Information.........................28
Section 13 References .......................................29
Section 14 Legal Notices ...................................29
10011164A.qxp 11/19/2007 9:54 AM Page IV
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