Bio-rad ProteinChip Qualification and Calibration Kits Instrukcja Użytkownika Pobierz pdf (Strona 15)

2.4 OQ Tests

2.4.1 Test 1 –– Detector Sensitivity

This test uses the ProteinChip detector qualification array to measure the signal-to-noise ratio

(S/N) of immunoglobubulin (IgG) at two different concentrations (10 fmol and 140 fmol).

Measurements are compared to a specification, and a pass/fail disposition is obtained.

Array Type ProteinChip detector qualification array

Usage Information Use once and discard; do not open until ready to use.

Recommended Testing Every other week

Frequency

Expected Testing Time 30 min to 1 hr

Pass/Fail Specification High concentration: pass if S/N is greater than 1,100

Low concentration: pass if S/N is greater than 5

1. Insert the ProteinChip detector qualification array into the instrument. If you are using an

Enterprise Edition instrument, place the array into any location within the cassette, fill the

remaining slots in the cassette with blank or used arrays, and place the cassette into the

instrument.

2. In ProteinChip data manager software, click Start and open the Protocol Mode tab.

3. Select Instrument > Preferences. In the Preferences dialog, click “…”. The Select Folder

dialog opens.

4. Select Projects > OQ_####/

Date

/Test 1 Detector Sensitivity, where #### is the serial

number of the instrument.

5. Run protocol “Test 1 Detector Sensitivity ” on partition 1 of 1 for all 8 spots on the array. Do

not change any values.

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