Bio-rad ProteinChip Qualification and Calibration Kits Instrukcja Użytkownika Pobierz pdf (Strona 26)

7. Plot the peaks with the filtering option turned off:

a. Select all 8 spectra.

b. Click the data analysis icon . The Analysis Settings dialog opens.

c. Open the Filtering tab and deselect the On checkbox.

d. Click OK.

8. Plot all 8 spectra. Using the peak selection tool, select all 7 peaks. Each spectrum should

resemble that shown below (intensities relative to each other may vary). Additional peaks

other than those indicated may consist of matrix, doubly-charged peaks, dimers or other

multimers, salt peaks, etc., as for any mass spectrum.

Peak Name Molecular Weight (Da)

1 Arg-8-vasopressin 1084.247

2 Somatostatin 1637.903

3 Dynorphin A 2147.5

4 ACTH 2933.5

5 Beta endorphin 3465

6 Arg-insulin 5963.8

7 Cytochrome C 12230.92

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Bio-rad ProteinChip Qualification and Calibration Kits Instrukcja Użytkownika Strona 26