Bio-rad ProteinChip Qualification and Calibration Kits Instrukcja Użytkownika Pobierz pdf (Strona 9)

Step 3

Set Up the

High Voltage (HV)

Conditioning Routine

1. Insert a blank ProteinChip array into the reader. If blank arrays are not

available, use expired arrays or arrays that have already been used.

a. For the Enterprise Edition instrument: place a cassette filled with

12 blank or used arrays into the instrument.

b. For the Personal Edition instrument: insert a blank ProteinChip

array.

2. In ProteinChip data manager software, select the instrument and click Start.

3. In the Protocol Mode tab, select Instrument > Quick Run.

4. Select all 8 spots, then set up a Quick Run protocol as shown below:

a. For the Enterprise Edition instrument:

RC3 HV pos 15 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 20 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 30 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV neg 15 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV neg 20 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV neg 25 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV neg 30 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 15 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 20 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 30 kV conditioning, partition 1 of 8, all 8 spots

RC3 HV pos 25 kV conditioning, partition 1 of 8, all 8 spots

b. For the Personal Edition instrument: