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ChromLab™ Software
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Bio-rad ChromLab™ Software Instrukcja Użytkownika Strona 223
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Comparing T
races
User Guide
|
221
Overlay View
All visible traces from all selected runs appear in a single chr
omatogram.
T
o switch between stacked and overlay views
Click Stack or Overlay on the Run tab toolbar or on the View menu.
1
2
...
218
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Chromatography Systems
1
Software
1
User Guide
1
NGC™ Chromatography
3
Systems and
3
User Guide
7
User Guide
9
Table of Contents
10
User Guide
11
1 Introduction
13
Main NGC Features
14
User Guide
15
NGC Chromatography Systems
16
User Guide
17
Finding Out More
18
2 The Workspace
19
The Home Window
20
User Guide
21
User Guide
23
The System Control Window
25
The Method Editor Window
26
The Evaluation Window
27
2
28
Touch Screen Menu Commands
29
Touch Screen Toolbar Commands
30
3 System Control
31
3
32
Manual Menu Commands
34
Tools Menu Commands
34
Context Menu Commands
35
Chromatogram View
37
Table 1. Trace Definitions
39
Trace Module Explanation
40
Showing or Hiding Traces
41
Changing Trace Color
42
Changing the Y-Scale Values
43
Autoscaling the UV Trace
43
To add annotations
45
To edit an annotation
45
To delete an annotation
45
User Guide
47
Fluidic Scheme Pane
48
User Guide
49
Sample Inject Valve
51
Sample Pump
52
Column Switching Valve
52
UV/Conductivity Monitors
52
Signal Import Module
52
Fluidic Scheme Configurations
53
User Guide
55
User Guide
57
Working with Fluidic Schemes
59
To change module settings
61
Fluidic Scheme Mapping
62
Color Label valve
63
To unmap valves
64
To cancel unmapping valves
64
To map valves
65
Calibrations
66
User Guide
67
Calibrating Pressure Settings
68
User Guide
69
To verify the plumbing path
70
System Settings
71
To set the delay volume
72
Orange 0.02" (0.5 mm)
73
Green 0.03" (0.75 mm)
73
Clear 0.062" (1.6 mm)
73
Control Flow Tab
74
To control the flow rate
75
Remote Access Tab
76
Trace Settings Tab
76
User Guide
77
Device Input Tab
78
User Guide
79
Device Output Tab
80
To disable the connection
81
Air Sensors Tab
82
To deactivate an air sensor
83
Email Notifications Tab
84
User Guide
85
System Name Tab
87
System Information
88
Detector Tab
89
Multi-Wavelength Detector
90
Preferences
91
User Guide
93
4 Performing a Manual Run
95
User Guide
97
User Guide
99
Preparing the System
101
User Guide
101
User Guide
103
Cleaning the System
104
Running an Experiment
105
User Guide
107
User Guide
109
To run an experiment step
110
Changing Module Settings
111
Stopping a Manual Run
111
Clearing Run Data
111
Saving a Manual Run
111
Viewing Run Data
113
5 Method Editor
115
Before You Begin
116
Opening a Method
117
5
118
File Menu Commands
119
Edit Menu Commands
120
View Menu Commands
121
Help Menu Commands
121
Toolbar Commands
121
Phase Library Pane
122
Adding a Phase to a Method
123
Method Outline Pane
124
Phase Parameters Pane
124
Method Steps Pane
125
To view step details
126
Method Settings Parameters
127
Column Selection
128
User Guide
129
To add user-defined columns
131
Detector Settings
133
Unit Selection
133
Fraction Collection
134
Buffer Selection
135
Pump Inlets
136
Pump + Inlet Valves
136
Pump + Buffer Blending
136
To select a buffer
137
Phase Controls and Parameters
138
User Guide
139
Standard Phases
140
User Guide
141
User Guide
143
Column Wash Parameters
145
Elution Parameters
146
Special Considerations
147
NGC Scout Systems
148
User Guide
149
User Guide
151
To clean the pH valves
152
User Guide
153
User Guide
155
System Preparation Parameters
156
Create New Phase Parameters
157
Step Library Pane
158
Scout Parameters Pane
159
6 Creating a Method
161
Standard Method Templates
162
User Guide
163
6
164
User Guide
165
User Guide
167
To create a method
168
To change the fluidic scheme
168
To specify general settings
169
User Guide
171
Adding Phases
172
Editing Methods and Phases
172
Renaming Phases
173
Rearranging Phases
173
Deleting Phases
174
User Guide
175
To run a method
177
To add runs to the run queue
177
Saving a Method
179
Renaming a Method
180
Deleting a Method
181
The Scouting Wizard
183
User Guide
185
User Guide
187
User Guide
189
Creating a Scouting Method
191
User Guide
193
Running a Scouting Method
194
To run a scout method
195
7 Evaluating Results
197
Managing Analysis Projects
198
To open a saved analysis
199
7
200
Analysis Menu Commands
203
Tab Toolbar Commands
204
Customizing the Chromatogram
205
Selecting the Active Trace
206
Showing or Hiding a Trace
207
Changing the Axes
207
Changing Trace Colors
208
Zooming In and Out
209
Chromatogram
210
To resize the selected region
211
Annotating the Chromatogram
212
Copying the Chromatogram
213
Changing Table Grouping
214
Sorting Table Columns
215
Copying a Table
216
User Guide
217
Managing Runs
218
To close a tab
219
To close all tabs
219
Comparing Traces
220
Stacked View
221
Overlay View
223
Hiding All Traces in a Group
224
Showing All Traces in a Group
224
To offset a trace
225
Managing Analyses
226
Copying an Analysis
227
Renaming an Analysis
227
Exporting Run Data
228
Peak Integration
229
Baseline Parameters
230
Peak Parameters
231
Peak Filtering
231
Default Parameters
231
Starting Peak Integration
232
User Guide
233
Changing Peak View Options
234
User Guide
235
Showing or Hiding Columns
237
Copying the Peaks Table
238
User Guide
239
Adjusting Peaks Manually
240
To add a peak
241
Saving Peak Integration Data
242
Evaluating Fractions
243
Rack Display
244
Outlet Valve Display
244
Fractions Table
245
User Guide
247
Viewing Fraction Details
248
Pooling Fractions
248
To create a fraction pool
249
To clear a pool
249
Viewing Heat Map Details
251
To hide the rack display
252
Column Performance Analysis
253
Calculate
254
Analyzing Column Performance
255
Click Delete
256
Column Performance Table
258
User Guide
259
8 Importing and Exporting
261
To import a method or run
262
Importing Unicorn Data Files
263
User Guide
265
Exporting Data
266
Exporting Data as an NGC File
267
To export only the method
268
To export only the run data
268
User Guide
269
To export multiple runs
270
User Guide
271
Exporting Diagnostic Logs
273
Change the date range
274
User Guide
275
9 Reports
277
Producing a Report
278
Method Reports
279
Run Reports
279
Printing a Report
280
Saving a Report
281
9
282
A Database Management
283
Back Up the NGC Database
284
Restore the NGC Database
285
A
286
B Multicolumn Purifications
287
Method base unit
288
Sample volume
288
Step duration and length
288
User Guide
289
Template Name Description
291
Plumbing the NGC System
293
Purification Templates
294
User Guide
295
Priming the NGC System
296
User Guide
297
Life Science
300
Bio-Rad
300
Laboratories, Inc
300
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