Bio-rad ChromLab™ Software Instrukcja Użytkownika Pobierz pdf (Strona 289)

Multicolumn Purification Method Templates

User Guide | 287

Table 7. Multicolumn sequential templates

Template Name Template Description

Affinity (1 ml) — Linear

Gradient

This method is used to purify many samples quickly. Using a

sample inlet valve and the sample pump, the method loads

sample onto separate affinity columns and washes unbound

material off in quick succession. This process can be repeated

for up to five columns. This method is designed to reduce the

target protein’s exposure to proteases. The target proteins are

then eluted from each column sequentially using a linear

gradient.

Affinity (1 ml) — Step (Manual

Load)

This method is used to purify many samples quickly. Using a

loop valve (a column switching valve plumbed to the injection

valve in the loop position with multiple sample loops), the

method loads sample onto separate af

finity columns and

washes unbound off material in quick succession. This

process can be repeated for up to five columns. This method

is designed to reduce the target protein’s exposure to

proteases. The target proteins are then eluted from each

column sequentially using a step gradient.

Affinity (1 ml) — Step Gradient This method is used to purify many samples quickly. Using a

sample inlet valve and the sample pump, the method loads

sample onto separate af

finity columns and washes unbound

material off in quick succession. This process can be repeated

for up to five columns. This method is designed to reduce the

target protein’s exposure to proteases. The target proteins are

then eluted from each column sequentially using a step

gradient.

Affinity (5 ml) — Linear

Gradient

This method is used to purify many samples quickly. Using a

sample inlet valve and the sample pump, the method loads

sample onto separate af

finity columns and washes unbound

material off in quick succession. This process can be repeated

for up to five columns. This method is designed to reduce the

target protein’s exposure to proteases. The target proteins are

then eluted from each column sequentially using a linear

gradient.

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