Bio-rad S3™ Cell Sorter Instrukcja Użytkownika Strona 122
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112 | S3 and S3e Cell Sorters
Troubleshooting
Table 30. Troubleshooting table (continued).
Error Possible Causes Troubleshooting Steps
Drastic light change Background movement in Redo QC and avoid changing light
front of green sliding door conditions in front of green sliding door
during QC process during the procedure
Sheath error The internal sheath The connections to the DI water and 8x
reservoir cannot maintain sheath fluid containers should be checked to
proper levels make sure they are connected, that they
are not empty, and that fluid can flow. It is
possible that their filters are clogged and
need to be replaced
Waste error Waste quick disconnect The connections to the waste container
not connected properly to should be checked to make sure they are
waste container connected and that waste can flow. Once
corrected, it will take time for the system to
Internal waste reservoir filled
clear the internal reservoir. You can observe
that pumping is happening by watching for
splashing in the waste container
Nozzle stage cannot return to Laser intersection cover has Adjust laser intersection cover so it is seated
proper position after nozzle been moved during tip swap correctly. Once in the proper place the
tip swap nozzle stage can be lowered into position
Cannot acquire sample. Room too cold or faulty Warm room to >63°F. If this does not help,
Hovering on acquisition button bubble detector contact service support
displays “bubble detected”
Arcing The high voltage deflection Stream spray or droplets are on the
plate monitor has measured deflection plates. Carefully clean and dry
the plate voltage deviating the plates
from normal
No data appear on the work- Nozzle interlock has been Open nozzle door all the way then close to
space after changing nozzle tip activated reset the laser interlock
No events during acquisition Nozzle door open Close the nozzle door
Laser shutter closed Cycle the nozzle door open then closed,
especially if cover was removed
Laser not on Turn on the laser in the user interface
Restart instrument and computer to correct
bad communication
Threshold too high Lower threshold
Triggering on noise Change the trigger parameter to FSC or SSC
Stream not centered in Use the electronic micromotors (Agillis)
laser beam positioner while acquiring to maximize event
signal — access within the system log file
No beads in sample Check sample tube, vortex
continues
- Cell Sorters 1
- Legal Notices 3
- Bio-Rad Technical Support 3
- Safety Warning Labels 5
- Instrument Warning Labels 5
- Regulatory Compliance 6
- Biohazards 6
- Table of Contents 9
- Introduction 11
- 1.1 System Components 12
- 1.2 Installation Requirements 12
- Hardware Description 13
- 2.1.1 Instrument Back Panel 14
- 2.1.2 Aerosol Evacuation Port 14
- 2.2 Fluidics System 15
- 2.2.2 Loading Stage 16
- Instruction Manual 17
- 2.2.3 Nozzle 18
- 2.2.4 Sort Collection Chamber 18
- 2.3 Optics 19
- 2.4 Electronics 21
- Software 23
- 3.1 Main Software Window 24
- 3.1.1 Title Bar Buttons 25
- 3.1.2 File Menu 25
- 3.1.3 Home Tab Toolbar 26
- 3.2 Control Panel 30
- 3.3 Administrator Tab Toolbar 32
- 3.3.1 Editing QC Criteria 33
- 3.3.2 Edit Droplets 34
- 3.3.3 Edit Streams 34
- Instruction Manual 35
- Test Pattern highlighted 35
- 3.3.4 User Reports 36
- 3.3.5 Managing Users 37
- 3.3.6 Decontaminate 38
- 3.3.8 Global Preferences 39
- Boost Adjustment 40
- Force Cleaning 40
- Default Plot Statistics 40
- Plot Defaults 40
- Parameter Names 41
- Default Save Location 41
- Emergency Contact Information 41
- Getting Started 43
- 4.2 Logging In 44
- 4.3 Daily Startup 45
- 4.4 Quality Control 46
- 4.4.1 Droplet Setup 47
- 4.4.2 Side Streams 48
- 4.4.3 Alignment 49
- 4.4.5 Drop Delay Maintenance 50
- 4.4.6 Recalculate Drop Delay 51
- 4.4.7 QC Criteria 51
- 4.4.8 QC Report 51
- 4.5 Protocols and Workspace 52
- 4.5.3 Saving Protocols 53
- 4.5.5 Opening FCS Files 54
- 4.5.6 Saving FCS Files 55
- 4.5.10 Changing Parameters 56
- 4.5.11 Viewing Statistics 57
- 4.5.13 Creating Regions 58
- 4.5.14 Creating Gates 59
- Acquisition 61
- 5.1.1 Target Event Rate 62
- 5.1.2 Event Limit 63
- 5.1.3 Cycle Mode 63
- 5.1.4 Trigger 63
- 5.1.7 Auto Save 64
- 5.1.5 Threshold 64
- 5.1.6 PMT Voltages 64
- 5.2 Compensation 65
- Instruction Manual 67
- Fig. 61. Compensation matrix 67
- 5.2.2 Manual Compensation 69
- 5.3.1 Hot Swap 70
- Instruction Manual 71
- 5.4 Optical Filters 72
- 5.4.2 Optical Filter Blocks 73
- 6.1 Sort Setup 75
- Fig. 70. Sort Logic window 76
- 6.1.1 Sort Collection 77
- 6.1.2 Volume Tracker 77
- 6.1.3 Setting Regions to Sort 78
- 6.2 Sort Modes 79
- 6.3 Sort Statistics 79
- 6.4 Sort Plots 81
- Additional Software Features 83
- 7.4 Clean System 84
- 7.5 Instrument Status Box 85
- 7.6 Status Bar 85
- 7.8 Quality Control Reports 86
- 7.7 Printing 86
- 7.9 User Reports 87
- 7.10 Biosafety System 88
- Shutdown 89
- Instruction Manual 91
- 8.1 Daily Shutdown 91
- Automatic Startup 93
- 10.2 Dealing with Clogs 98
- Maintenance 100
- 10.4 Swap Nozzle Tip Wizard 101
- Instruction Manual 101
- Instruction Manual 103
- Instruction Manual 105
- Cleaning the nozzle tip: 108
- Sonication: 108
- Syringe wash: 108
- Instruction Manual 109
- Instruction Manual 111
- Instruction Manual 113
- 10.5 Optical Filter Cleaning 115
- 10.6 Disinfectants 115
- 10.7 Decontamination 115
- Instruction Manual 119
- Troubleshooting 121
- References 125
- and S3e 127
- Cell Sorter 127
- Specifications 127
- Ordering Information 129
- Life Science 132
- Bio-Rad 132
- Laboratories, Inc 132
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