Bio-rad S3™ Cell Sorter Instrukcja Użytkownika Strona 9
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Instruction Manual | vii
Table of Contents
Chapter 1: Introduction 1
1.1 System Components 2
1.2 Installation Requirements 2
Chapter 2: Hardware Description 3
2.1 System Overview 3
2.2 Fluidics System 5
2.3 Optics 9
2.4 Electronics 11
Chapter 3: ProSort
™
Software 13
3.1 Main Software Window 14
3.2 Control Panel 20
3.3 Administrator Tab Toolbar 22
Chapter 4: Getting Started 33
4.1 Checking Bulk Fluidics 33
4.2 Logging In 34
4.3 Daily Startup 35
4.4 Quality Control 36
4.5 Protocols and Workspace 42
Chapter 5: Acquisition 51
5.1 Acquisition Setup 51
5.2 Compensation 55
5.3 Checking or Swapping Fluidics 60
5.4 Optical Filters 62
Chapter 6: Sorting 65
6.1 Sort Setup 65
6.2 Sort Modes 69
6.3 Sort Statistics 69
6.4 Sort Plots 71
Chapter 7: Additional Software Features 73
7.1 Debubble 73
7.2 Unclog 73
7.3 Swap Tip 73
7.4 Clean System 74
7.5 Instrument Status Box 75
7.6 Status Bar 75
7.7 Printing 76
7.8 Quality Control Reports 76
7.9 User Reports 77
7.10 Biosafety System 78
Chapter 8: Shutdown 79
8.1 Daily Shutdown 79
Chapter 9: Automatic Startup 83
9.1 Scheduling an Automatic Startup 83
9.2 Previously Scheduled Automatic Startups 84
Chapter 10: Maintenance 87
10.1 General Maintenance 87
10.2 Dealing with Clogs 88
10.3 Cleaning or Replacing Nozzle Tip 88
10.4 Swap Nozzle Tip Wizard 89
10.5 Optical Filter Cleaning 105
10.6 Disinfectants 105
10.7 Decontamination 105
Troubleshooting 111
References 115
S3 and S3e Cell Sorter Specifications 117
Ordering Information 119
- Cell Sorters 1
- Legal Notices 3
- Bio-Rad Technical Support 3
- Safety Warning Labels 5
- Instrument Warning Labels 5
- Regulatory Compliance 6
- Biohazards 6
- Table of Contents 9
- Introduction 11
- 1.1 System Components 12
- 1.2 Installation Requirements 12
- Hardware Description 13
- 2.1.1 Instrument Back Panel 14
- 2.1.2 Aerosol Evacuation Port 14
- 2.2 Fluidics System 15
- 2.2.2 Loading Stage 16
- Instruction Manual 17
- 2.2.3 Nozzle 18
- 2.2.4 Sort Collection Chamber 18
- 2.3 Optics 19
- 2.4 Electronics 21
- Software 23
- 3.1 Main Software Window 24
- 3.1.1 Title Bar Buttons 25
- 3.1.2 File Menu 25
- 3.1.3 Home Tab Toolbar 26
- 3.2 Control Panel 30
- 3.3 Administrator Tab Toolbar 32
- 3.3.1 Editing QC Criteria 33
- 3.3.2 Edit Droplets 34
- 3.3.3 Edit Streams 34
- Instruction Manual 35
- Test Pattern highlighted 35
- 3.3.4 User Reports 36
- 3.3.5 Managing Users 37
- 3.3.6 Decontaminate 38
- 3.3.8 Global Preferences 39
- Boost Adjustment 40
- Force Cleaning 40
- Default Plot Statistics 40
- Plot Defaults 40
- Parameter Names 41
- Default Save Location 41
- Emergency Contact Information 41
- Getting Started 43
- 4.2 Logging In 44
- 4.3 Daily Startup 45
- 4.4 Quality Control 46
- 4.4.1 Droplet Setup 47
- 4.4.2 Side Streams 48
- 4.4.3 Alignment 49
- 4.4.5 Drop Delay Maintenance 50
- 4.4.6 Recalculate Drop Delay 51
- 4.4.7 QC Criteria 51
- 4.4.8 QC Report 51
- 4.5 Protocols and Workspace 52
- 4.5.3 Saving Protocols 53
- 4.5.5 Opening FCS Files 54
- 4.5.6 Saving FCS Files 55
- 4.5.10 Changing Parameters 56
- 4.5.11 Viewing Statistics 57
- 4.5.13 Creating Regions 58
- 4.5.14 Creating Gates 59
- Acquisition 61
- 5.1.1 Target Event Rate 62
- 5.1.2 Event Limit 63
- 5.1.3 Cycle Mode 63
- 5.1.4 Trigger 63
- 5.1.7 Auto Save 64
- 5.1.5 Threshold 64
- 5.1.6 PMT Voltages 64
- 5.2 Compensation 65
- Instruction Manual 67
- Fig. 61. Compensation matrix 67
- 5.2.2 Manual Compensation 69
- 5.3.1 Hot Swap 70
- Instruction Manual 71
- 5.4 Optical Filters 72
- 5.4.2 Optical Filter Blocks 73
- 6.1 Sort Setup 75
- Fig. 70. Sort Logic window 76
- 6.1.1 Sort Collection 77
- 6.1.2 Volume Tracker 77
- 6.1.3 Setting Regions to Sort 78
- 6.2 Sort Modes 79
- 6.3 Sort Statistics 79
- 6.4 Sort Plots 81
- Additional Software Features 83
- 7.4 Clean System 84
- 7.5 Instrument Status Box 85
- 7.6 Status Bar 85
- 7.8 Quality Control Reports 86
- 7.7 Printing 86
- 7.9 User Reports 87
- 7.10 Biosafety System 88
- Shutdown 89
- Instruction Manual 91
- 8.1 Daily Shutdown 91
- Automatic Startup 93
- 10.2 Dealing with Clogs 98
- Maintenance 100
- 10.4 Swap Nozzle Tip Wizard 101
- Instruction Manual 101
- Instruction Manual 103
- Instruction Manual 105
- Cleaning the nozzle tip: 108
- Sonication: 108
- Syringe wash: 108
- Instruction Manual 109
- Instruction Manual 111
- Instruction Manual 113
- 10.5 Optical Filter Cleaning 115
- 10.6 Disinfectants 115
- 10.7 Decontamination 115
- Instruction Manual 119
- Troubleshooting 121
- References 125
- and S3e 127
- Cell Sorter 127
- Specifications 127
- Ordering Information 129
- Life Science 132
- Bio-Rad 132
- Laboratories, Inc 132
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