Bio-rad S3™ Cell Sorter Instrukcja Użytkownika Pobierz pdf (Strona 29)

Instruction Manual | 19

3.1 Main Software Window

Table 17. Fluidics buttons and their functions.

Button Name Function

Debubble Removes bubbles from nozzle

Unclog Allows user to pull vacuum in the case of a nozzle clog. Use this option before

removing the tip for sonication or replacing with a new nozzle tip

Swap Tip Stops the sheath ﬂow to allow nozzle tip replacement. A wizard will appear to

walk the user through a nozzle change. Once the tip has been replaced, QC

needs to be rerun to verify alignment and set the drop delay

Swap Fluidics Allows ﬂuidic containers to be swapped without shutting the system down.

Prepare a full replacement or empty container prior to pressing this button, as

a three-minute timer starts after the button is pressed

Clean Cleaning can be done at low or high pressure. High pressure cleaning will cause

the system to stop maintaining droplets and a QC procedure must be run before

sorting. If cleaning between samples, the low pressure option is recommended

Table 18. Other buttons and their functions.

Button Name Function

Annotation Adds an annotation window to the workspace. Annotations added prior to

saving will be saved to the FCS ﬁle’s embedded protocol

Image Allows an image to be added to the workspace. The image can be moved

or deleted. If added prior to saving, this image will be saved to the FCS ﬁle’s

embedded protocol

Basic Adds basic information to the print page in the form of a header. This information

Information includes date, time, user login, serial number, ﬁlename, event source. If added

prior to saving, this information will be saved to the FCS ﬁle’s embedded protocol

Filter Conﬁguration Adds the optical layout to the print page showing PMT and optical ﬁlter setup.

This can be modiﬁed to match the actual ﬁlters if they have been changed. If

added prior to saving, this will be saved to the FCS ﬁle’s embedded protocol

Table 19. Publish and user buttons and their functions.

Button Name Function

Preview Displays a preview of the page layout(s) setup prior to printing

Page Orientation Changes between a printable landscape or portrait workspace

Print Opens the print dialog to select printer, number of copies, etc. and print.

Note: Use the print preview option to see where page breaks occur

Print Options Adds additional windows or instrument settings to the page layout for printing.

Change Password Allows the currently logged-in user to change password

System Log Opens window for system log. Displays date, time, error, error details. This

option is helpful when working with technical support and service engineers

for troubleshooting

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