Bio-rad ChromLab™ Software Instrukcja Użytkownika Pobierz pdf (Strona 189)

Mixer

Instrument Guide | 187

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Chromatography Systems 1
Software 1
Instrument Guide 1
NGC™ Chromatography 3
Systems and 3
Instrument Guide 7
Instrument Guide 9
Table of Contents 10
Instrument Guide 11
1 Introduction 13
(on the NGC Quest 14
Plus system) 14
Main Features 15
Site Requirements 16
Environment 17
Bench Space 17
Safety Requirements 18
Finding Out More 19
1 20
2 The NGC Instrument 21
2 22
Instrument Guide 23
Front View 24
Instrument Guide 25
Back View 26
Right Side View 27
Left Side View 28
System Pumps 31
Detailed Attributes 32
System Pump LEDs 32
How the System Pumps Function 32
Pump Priming Port 33
Piston Wash System 34
Sample Pump 35
Sample Pump LEDs 36
How the Sample Pump Works 36
Instrument Guide 37
Sample Inject Valve 39
Port Function 40
Sample Inject Valve LEDs 41
Valve Position Explanation 42
Buffer Blending Valve LEDs 46
Buffer Blending Mode 47
Gradient Mode 47
Inlet Valves 48
Inlet Valve 49
Ports on the Inlet Valves 50
Inlet Valve LEDs 51
How the Inlet Valves Work 52
Outlet Valve 53
Ports on the Outlet Valve 54
Outlet Valve LEDs 55
How the Outlet Valve Works 56
Column Switching Valve LEDs 60
Instrument Guide 61
Instrument Guide 63
Detectors 66
Instrument Guide 67
Single-Wavelength UV Detector 68
Instrument Guide 69
Conductivity Detector 71
How They Work 72
The Backpressure Regulator 73
Ports on the pH Detector 75
How the pH Detector Works 76
Instrument Guide 77
Pressure Rating 78
Mounted 78
Sample Injection Loops 79
Fittings 80
LEDs and LED Screens 81
Air Sensors 82
Instrument Guide 83
Air Sensor LEDs 84
Pressure Sensors 85
Temperature Sensor 85
NGC Touch Screen 86
Buffer Tray 87
Product Configurations 88
Discover series 89
3 Preparing the Instrument 91
Module Review 92
Instrument Guide 93
NGC Systems Accessories Kit 94
NGC Scout Accessories Kit 95
NGC Discover Accessories Kit 95
Buffer Bottles 96
Waste Bottles 96
Instrument Guide 97
Preparing the Detectors 99
Changing the Mixer Barrel 100
Barrel Size Flow Rate, ml/min 101
F10 Pump F100 Pump 101
(air sensor to pump inlet) 103
To activate the air sensors 105
Instrument Guide 107
Instrument Guide 109
Instrument Guide 111
Instrument Guide 113
Trigger wire 115
Positive 115
Starting the NGC Instrument 116
Instrument Guide 117
To verify the plumbing path 119
To prime the system pumps 121
To purge the system pumps 122
Instrument Guide 123
Priming the NGC Scout System 124
Instrument Guide 125
Purging the NGC Scout System 126
Instrument Guide 127
Priming NGC Discover Systems 128
Instrument Guide 129
Instrument Guide 131
To prime the sample pump 132
Instrument Guide 133
Instrument Guide 135
Instrument Guide 137
To purge the sample pump 138
Instrument Guide 139
Instrument Guide 141
Preparing the Columns 142
Instrument Guide 143
 UV monitor 144
Washing the Columns 145
Calibrating pH 147
To zero the pressure 148
Instrument Guide 149
A Maintaining the Instrument 151
Storing the NGC Instrument 153
Interval Component Action 154
As Needed 156
Component Solution to use 157
System and Sample Pumps 158
Pump Head Assembly 159
Piston Wash Assembly 160
Disassembling the Pumps 161
Instrument Guide 163
To clean the pump heads 164
Instrument Guide 165
Instrument Guide 167
To replace the pump head seal 169
Removing the Wash Housing 171
Removing the Piston 172
Instrument Guide 173
Instrument Guide 175
Reassembling the Pumps 177
Installing the Wash Housing 178
Instrument Guide 179
Installing the Pump Heads 180
Instrument Guide 181
Instrument Guide 183
Instrument Guide 185
Conditioning New Seals 187
Instrument Guide 189
Disassembling the Mixer 190
Instrument Guide 191
To change the O-rings 193
To clean the mixer body 195
Reassembling the Mixer 196
Instrument Guide 197
The Detectors 198
Instrument Guide 199
Instrument Guide 201
Instrument Guide 203
Instrument Guide 205
Instrument Guide 207
Back of LED Board 209
Front of LED Board 209
To reset lamp time details 210
Instrument Guide 211
6. Remove the lamp: 213
Instrument Guide 215
Instrument Guide 217
Storing the pH Electrode 219
Cleaning the pH Electrode 219
Replacing the pH Probe 220
Instrument Guide 221
Other Components 222
Instrument Guide 223
Fourth expansion tier 224
Previously attached 224
For example: 225
Instrument Guide 227
Task: Refer to the section: 228
Instruments 229
Bottom of Module 231
Top of Module 231
Instrument Guide 233
Instrument Guide 235
Cutting Replacement Tubing 236
Instrument Guide 237
Instrument Guide 239
Instrument Guide 241
Instrument Guide 243
Instrument Guide 245
B Troubleshooting the 247
Instrument 247
Instrument Guide 249
 Perform CIP to clean the 250
 Replace the pump seals 250
 Replace the check valves 250
Instrument Guide 251
 Replace check valves 252
Instrument Guide 253
UV or UV/Vis Detector 255
 Use the lamp usage values 256
 See Replacing the Single 256
 See Replacing the Multi 256
Instrument Guide 257
 Recalibrate both system 258
Instrument Guide 259
Conductivity Monitor 260
Instrument Guide 261
Buffer Blending Valve 262
Column Switching Valve 262
External Devices 263
C Plumbing the System 265
Plumbing NGC Systems 266
Plumbing All NGC Systems 267
System Pumps Wash Flow 268
Sample Pump Wash Flow 268
Instrument Guide 269
To plumb the sample pump 270
Instrument Guide 271
C 272
Plumbing the NGC Quest System 273
To plumb the system pumps 275
Plumbing the NGC Scout System 276
Instrument Guide 277
To plumb the pH valve 278
Instrument Guide 279
Instrument Guide 281
Tubing color Port Solution 282
Instrument Guide 285
Plumbing Sample Inlet Valves 287
Plumbing Outlet Valves 288
Instrument Guide 289
D Connecting the C-96 291
Instrument Guide 293
To prepare the tubing 294
Autosampler 295
Sample Inject 295
Instrument Guide 297
E Regulatory Information 299
5 ml F10 2,650 psi 300
5 ml F100 1,400 psi 300
DynaLoop™ 25 ml F100 700 psi 300
DynaLoop 90 ml F100 270 psi 300
FCC Warning and Notes 301
F NGC Chromatography 303
Systems Catalog Numbers 303
Life Science 306
Bio-Rad 306
Laboratories, Inc 306

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