Bio-rad ChromLab™ Software Instrukcja Użytkownika Pobierz pdf (Strona 254)

B | Troubleshooting the Instrument

252 | NGC Chromatography Systems and ChromLab Software

Backpressure values

are higher or lower than

expected (continued).

The inline filter is clogged. Inspect the inline filter and

replace if necessary.

Note: Recalibrate after

stopping the pumps. In

ChromLab, select Tools >

Calibrate > Pump Flow

Rate. Complete the

calibration steps in the

dialog box.

Unintended increase in flow rate. In ChromLab, verify that the

flow rate setting is correct.

Particulate matter has

accumulated in the system,

causing a resistance to flow.

Tip: Prevent or minimize

high backpressure by filtering

buffers and samples and by

changing the pump seals

before they deteriorate

completely.

Isolate the blockage in the

system tubing. Loosen the

connection fittings at the

detector and work backwards

toward the column and pump

head.

The rinse fluid is not

circulating.

The tubing lines are not connected

or the fittings are not tight.

Ensure that the wash system

tubing lines are connected

properly and reprime the

system.

The rinse fluid reservoir

is overflowing.

Fluid is leaking past the pump

head seals into the wash system.

Replace the pump head seal.

Symptom Possible Cause Possible Solution

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